Prevention of ischemia-reperfusion injury in rat ovarian tissue with the on-off method.
نویسندگان
چکیده
Ischemia is defined as cell death caused by insufficient perfusion of the tissue due to reduction in arterial or venous blood flow, depletion of cellular energy storages, and accumulation of toxic metabolites. The positive effects of controlled reperfusion are known and are used clinically. But the positive effects of controlled reperfusion on ovarian tissue have not been seen in the literature yet. The biochemical and histopathological comparative investigation of rat ovaries that were experimentally exposed to ischemia (IG), ischemia-reperfusion (I/R), and ischemia-controlled reperfusion (ICR) was aimed. Forty rats were divided into four groups (10 rats per group). First group: 3 h ischemia by vascular clips on ovarian tissue. Second group: 3 h ischemia + 1 h reperfusion. Third group: 3 h ischemia + 1 h controlled reperfusion (on-off method: controlled reperfusion by opening and closing the clips (on/off) in 10-second intervals, for 5 times for a total of 100 seconds). Fourth group: healthy rats. Biochemical (tGSH, MDA, and DNA damage level and SOD activity) and histopathological analysis were performed. The highest glutathione and superoxide dismutase measurements were found in ischemia/controlled reperfusion group among the ischemia or ischemia/reperfusion groups. Similarly the damage indicators (malondialdehyde, DNA damage level and histopathological damage grade) were the lowest in ischemia/controlled reperfusion group. These results indicate that controlled reperfusion can be helpful in minimizing ischemia-reperfusion injury in ovarian tissue exposed to ischemia for various reasons (ovarian torsion, tumor, etc.).
منابع مشابه
Bilateral ovarian ischemia/reperfusion injury and treatment options in rats with an induced model of diabetes
Objective(s):This study investigated the effects of melatonin, famotidine, mirtazapine, and thiamine pyrophosphate on ischemia/reperfusion (I/R) injury in diabetic rats and evaluated oxidant and antioxidant marker measurement results. It also examined the effects of the drugs aimed at preventing infertility that may result from I/R injury. Materials and Methods: Diabetic rats were divided into ...
متن کاملAntioxidant and antiapoptotic effects of erdosteine in a rat model of ovarian ischemia-reperfusion injury
Objective(s): To evaluate the protective effect of erdosteine, an antiapoptotic and antioxidant agent, on torsion–detorsion evoked histopathological changes in experimental ovarian ischemia-reperfusion (IR) injury. Materials and Methods: Eighteen female Wistar albino rats were used in control, IR, and IR+Edosteine (IR-E) groups, (n=6 in each). The IR-E group received the erdosteine for seven da...
متن کاملThe Study of Petoxifylline Drug Effects on Renal Apoptosis and Bcl2 Gene Expression Changes Following Ischemic Reperfusion Injury in Rat
Background & Target: Ischemia Reperfusion injury is the tissue damage caused when blood supply returns to the tissue after a period of ischemia or lack of oxygen. In this study, the effect of pentoxyfylline on bcl2 gene expression changes and cell injury in kidney of rat following Ischemia Reperfusion were evaluated.Methods: In this experimental study, 20 male wistar rats with average weight of...
متن کاملThe Study of Petoxifylline Drug Effects on Renal Apoptosis and Bcl2 Gene Expression Changes Following Ischemic Reperfusion Injury in Rat
Background & Target: Ischemia Reperfusion injury is the tissue damage caused when blood supply returns to the tissue after a period of ischemia or lack of oxygen. In this study, the effect of pentoxyfylline on bcl2 gene expression changes and cell injury in kidney of rat following Ischemia Reperfusion were evaluated.Methods: In this experimental study, 20 male wistar rats with average weight of...
متن کاملBiological and Histopathological Investigations of Moclobemide on Injured Ovarian Tissue Following Induction of Ischemia-Reperfusion in Rats
متن کامل
Effect of Systemic Administration of Curcumin on Ischemia-Reperfusion Injury in Ovaries: An Animal Model Study
Objective-Ovarian torsion must be diagnosed and treated as much early as possible. The aim of the present study was to investigate effects of interaperitoneal administration of curcumin on ischemia-reperfusion injury in ovaries. Design- Experimental Study Animals- Twent-four healthy female Wistar rats Procedures- Twent-four healthy female Wistar rats weighing approximately 260...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Journal of physiology and pharmacology : an official journal of the Polish Physiological Society
دوره 62 5 شماره
صفحات -
تاریخ انتشار 2011